This constant high rate of ROS production leads rapidly to extreme macromolecular oxidation, here it is observed in the AOPP and MDA detected after 3 h in samples treated with bare P25TiO₂NPs (Fig. 6, Fig. 7). Macromolecular oxidation includes, among others, both protein and lipid oxidation. The ROS causes protein oxidation by direct reaction or indirect reactions with secondary by-products of oxidative stress. Protein fragmentation or cross-linkages could be produced after the oxidation of amino acid side chains and protein backbones. These and later dityrosine-containing protein products formed during excessive production of oxidants are known as advanced oxidation protein products (AOPP). They absorb at 340 nm and are used to estimate the damage to structural cell amino acids. Lipid oxidation is detected by the conjugation of oxidized polyunsaturated lipids with thiobarbituric acid, forming a molecule that absorbs light at 532 nm. Polyunsaturated lipids are oxidized as a result of a free-radical-mediated chain of reactions. The most exposed targets are usually membrane lipids. The macromolecular damage could represent a deadly danger if it is too extensive, and this might be the case. Moreover, it could be observed that cellular damage continues further and becomes irrevocable after 6 h and MDA could not be detected. This may be due to the fact that the lipids were completely degraded and cells were no longer viable. Lipids from the cell membrane are the most prone to oxidation. In fact, lipid peroxidation biomarkers are used to screen the oxidative body balance [51]. At the same time, AOPP values are up to 30 times higher for bare nanoparticles in comparison to the functionalized ones.

Anatase titanium dioxide (TiO2) is a key inorganic compound renowned for its exceptional photocatalytic properties, stability, and versatility in various applications. Among the various crystalline forms of titanium dioxide, anatase is particularly favored in industries ranging from paints and coatings to cosmetics and solar cells. The rise of anatase titanium dioxide manufacturers is a testament to the compound’s increasing importance in modern technology and environmental applications.

What other candies and food contain titanium dioxide?

The additives that received a “no safety concern” conclusion based on current estimated dietary exposure are as follows:

TiO₂ is typically thought of as being chemically inert, meaning it does not react with other chemicals and is, therefore, a stable substance that can be used in many different industries and for various applications.